DNA fragmentation characteristic of programmed cell death (PCD) detected in NaCl
Author
Svetlana Shishkova Universidad Nacional Aut
Keywords
DNA fragmentation characteristic, programmed cell death, Stenocereus gummosus
Description
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DNA fragmentation characteristic of programmed cell death (PCD) was detected
in NaCl-treated determinate primary roots of Stenocereus gummosus (Cactaceae)
using the terminal deoxynucleotide transferase-mediated dUTP nick-end labeling
(TUNEL) assay. Nuclei in the bottom left image, a longitudinal section of a
root tip after PCD-inducing NaCl treatment, fluoresce blue after 4'-6-diamidino-2-phenylindole
(DAPI) staining. Those nuclei undergoing DNA fragmentation fluoresce green in
the same section (top left, fluorescein-isothiocyanate [FITC] staining). During
root development, PCD was not involved in meristem exhaustion, but did occur
in root-hair and root-cap cells. Both DNA and nuclear fragmentation were detected
in a root-hair cell (right: top, FITC; middle, DAPI). Dead remnants (red-fluorescing
nuclei, propidium-iodine stain, bottom right) of the root cap lie below a root
that has terminated growth.
Publisher
Botanical Society of America
Additional data
copyright: Svetlana Shishkova, Joseph G. Dubrovsky, BSA
license: http://images.botany.org/index.html#license